Detection of bacterial biofilm on stainless steel by hyperspectral fluorescence imaging
Summary:
In this study, hyperspectral fluorescence imaging
techniques were investigated for detection of microbial
biofilm on stainless steel plates typically used to manufacture
food processing equipment. Stainless steel coupons were
immersed in bacterium cultures of nonpathogenic E. coli,
Pseudomonas pertucinogena, Erwinia chrysanthemi, and Listeria
innocua.
Following a 1-week growth in rich medium
tryptic soy broth (TSB) and M9 minimal medium with casamino
acids (M9C), biofilm formations were evaluated using a
recently developed portable hyperspectral fluorescence
imaging system. Hyperspectral fluorescence images of the
biofilm samples, in response to ultraviolet-A (320 to
400 nm) excitation, were acquired from 416 to 700 nm.
Fluorescence images in the blue emission peak region exhibited
the most contrast between biofilms and stainless steel
coupons. On the basis of correlation analyses, two-band
ratios compared with the single-band images enhanced the
contrast between the biofilm forming area and untreated
coupon surfaces. A two-band fluorescence ratio image,
444/588 nm, resulted in the greatest contrast between
the biofilm formations and stainless steel coupon for
the biofilms grown in M9C medium. TSB medium showed relatively
high auto fluorescence, and thus further investigation
is needed to mitigate the contribution of strong TSB auto-fluorescence
in detection of biofilms.
Format:
Pages :6
Size: 580 kb
Author :Won Jun, Ph.D.,Kangjin Lee, Ph.D. ,Patricia
Millner, Ph.D.
Manan Sharma, Ph.D. ,Kuanglin Chao, Ph.D.,Moon S. Kim,
Ph.D.
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